Widal Test Procedure |How Typhoid is Diagnosed #lab #laboratory #cls #mls #medtechstudent #shortvide

The Widal Test is a serological test used to diagnose typhoid fever, a disease caused by Salmonella typhi and Salmonella paratyphi. It detects the presence of antibodies in a patient’s blood that react to antigens of these bacteria. Below is a detailed explanation of the procedure and purpose: Purpose of the Widal Test The test is performed to: • Detect S. typhi and S. paratyphi infections in patients showing symptoms such as fever, abdominal pain, and weakness. • Monitor typhoid fever in regions where it is endemic. Materials Needed • Patient’s serum sample (blood sample processed to separate serum). • Antigen suspensions: • O antigen: from the bacterial cell wall. • H antigen: from bacterial flagella. • Test tubes or microtiter plates. • Saline solution for dilution. • Pipettes. Procedure of the Widal Test 1. Blood Collection: • A venous blood sample is drawn from the patient. • The serum is separated by centrifugation. 2. Dilution: • The patient’s serum is diluted with saline in a series of test tubes or wells. 3. Antigen Addition: • The O and H antigen suspensions are added to the diluted serum samples. 4. Mixing and Incubation: • The mixtures are gently shaken and incubated at 37°C for about 1-2 hours. 5. Observation: • The samples are checked for agglutination (clumping). • Agglutination indicates a reaction between antibodies in the serum and the bacterial antigens. Interpretation of Results • Positive Test: Agglutination indicates the presence of antibodies against S. typhi or S. paratyphi. • A significant rise in antibody titers (e.g., 1:160 or above) suggests infection. • Negative Test: No agglutination means no detectable antibodies, or the infection is not typhoid. Limitations • False positives may occur due to previous infections or vaccinations. • Results need clinical correlation and, if possible, a repeat test after a week to check rising titers. 1. “What is the Widal Test? Typhoid Fever Diagnosis Made Simple” 2. “Widal Test Procedure: How Typhoid is Diagnosed” 3. “Typhoid Fever Test: Understanding the Widal Test Process” 4. “Widal Test Explained: Diagnosis, Procedure, and Results” 5. “Diagnosing Typhoid Fever: Everything About the Widal Test” 6. “Widal Test for Typhoid Fever | Easy-to-Understand Guide” 7. “How the Widal Test Detects Typhoid Fever” 8. “Typhoid Test Made Simple: Widal Test Explained Step by Step” General Health & Medical Hashtags: • #WidalTest • #typhoidfever • #MedicalTests • #HealthAwareness • #DiagnosticTests • #HealthcareTips • #MedicalEducation • #LabTests • #TyphoidDiagnosis • #MedicalExplained • #HealthMatters • #MedicalKnowledge • #EasyHealthGuide • #healthexplained • #CreedAndDeed • #MedicalInsights • #WidalTestProcedure • #HealthSimplified • #TyphoidTreatment