
Creating 0.9% Normal Saline Solution: A Step-by-Step Guide।Normal Saline । Heredity Biosciences
0.9% Saline Solution Preparation Introduction: 0.9% Saline Solution, often referred to as Normal Saline, is a fundamental solution in medical and laboratory practices. It is an isotonic solution containing 9 grams of sodium chloride (NaCl) dissolved in distilled water to make 1 liter of solution. This concentration matches the osmolarity of human blood and is widely used for various applications, including intravenous fluid replacement and laboratory procedures. Materials: Sodium chloride (NaCl) - 9 grams Distilled water - 1 liter Sterile container or bottle Stirring rod Weighing balance Autoclave (for sterilization) Procedure: Begin by measuring 9 grams of sodium chloride (NaCl) using a weighing balance. Ensure that the measurement is precise. Pour the 9 grams of NaCl into a sterile container or bottle. Add 1 liter of distilled water to the container with NaCl. Use caution to avoid contamination, and ensure that the water is at room temperature. Gently stir the solution with a sterile stirring rod until all the sodium chloride has completely dissolved. This may take a few minutes. Once the solution is prepared, it should be sterilized. The entire container or bottle, including its cap, should be autoclaved to ensure sterility. After autoclaving, allow the solution to cool to room temperature. Usage: 0.9% Saline Solution is now ready for use in medical procedures, laboratory experiments, or any application where an isotonic saline solution is required. Note: Always follow standard aseptic techniques and safety guidelines when preparing and handling saline solutions. ABOUT HEREDITY BIOSCIENCES.... Heredity Biosciences Leading Biotechnology company and it is providing research services to Hospitals, life sciences Institutes and Pharma companies as well as training for students. We are providing students, complete hands-on procedure for biotechnological / biomedical training. More Details visit https://hereditybio.in/training Course Modules: https://drive.google.com/drive/folder... More Information Connect: 7381298980 Latest research activities join our telegram channel: https://t.me/+VPMjLUTSoGhk7eft Research Activities Photos: https://goo.gl/maps/S6qgaxFfhHNh6odN9 Heredity Biosciences Plot No:818, Mayfair Lagoon Road, Jayadev Vihar, Bhubaneswar Email: hereditybioacademy@com/7381298980